IPP Complex Reinforces Adhesion by Relaying Tension-Dependent Signals to Inhibit Integrin Turnover

• DOI: 10.1016/j.celrep.2016.02.052
• Publication Date: 2016-03-10T17:25:47Z
• Authors: Katerina M. Vakal...; Georgios Chrysanthis; Maria Anna Rapsom...; Zoi Lygerou; Christos G. Zervas
• Keywords: Tension (geology)

Cytoskeleton-mediated forces regulate the assembly and function of integrin adhesions; however, underlying mechanisms remain unclear. The tripartite IPP complex, comprising ILK, Parvin, PINCH, mediates integrin-actin link at Drosophila embryo muscle attachment sites (MASs). Here, we demonstrate a developmentally earlier for complex: to reinforce integrin-extracellular matrix (ECM) adhesion in response tension. In IPP-complex mutants, integrin-ECM linkage MASs breaks intense contractility. Mechanistically, complex is required relay force-elicited signals that decelerate turnover plasma membrane so immobile fraction adequate withstand Epistasis analysis shows alleviation contractility, downregulation endocytosis, enhanced binding ECM are sufficient restore maintain integrin-adhesome organization mutants. Our findings reveal role as an essential mechanosensitive regulatory switch vivo.