Comprehensive Proteomic Analysis of Human Erythropoiesis

• DOI: 10.1016/j.celrep.2016.06.085
• Publication Date: 2016-07-21T22:27:13Z
• Authors: Emilie-Fleur Gautier; Sarah Ducamp; Marjorie Leduc; Virginie Salnot; François Guillonneau; Michael Dussiot; John Hale; Marie-Catherine G...; Anna Raimbault; Luc Douay; Catherine Lacombe; Narla Mohandas; Frédérique Verdier; Yael Zermati; Patrick Mayeux
• Keywords: Reticulocyte; Proteome; Erythroblast

Mass spectrometry-based proteomics now enables the absolute quantification of thousands proteins in individual cell types. We used this technology to analyze dynamic proteome changes occurring during human erythropoiesis. quantified expression 6,130 erythroid differentiation from late burst-forming units-erythroid (BFU-Es) orthochromatic erythroblasts. A modest correlation between mRNA and protein was observed. identified several with unexpected patterns cells, highlighting a breakpoint process at basophilic stage. also distribution reticulocytes pyrenocytes after enucleation. These analyses that are actively sorted either reticulocyte or pyrenocyte. Our study provides complex cellular humans, it establishes framework for future studies disordered