Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

• DOI: 10.1016/j.celrep.2016.07.005
• Publication Date: 2016-07-29T14:46:35Z
• Authors: Rotem Ben-Tov Perry; Ida Rishal; Ella Doron-Mandel; Ashley L. Kalinski; Katalin F. Medzih...; Marco Terenzio; Stefanie Alber; Sandip Koley; Albina Lin; Meir Rozenbaum; Dmitry Yudin; Pabitra K. Sahoo; Cynthia Gomes; Vera Shinder; Wasim Geraisy; Eric A. Huebner; Clifford J. Woolf; Avraham Yaron; Alma L. Burlingame; Jeffery L. Twiss; Mike Fainzilber
• Keywords: Nucleolin; Importin; Growth cone

How can cells sense their own size to coordinate biosynthesis and metabolism with growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length cell sensing, but the nature of motor-transported signals remained elusive. Here, we show that mRNA localization regulates neuronal cycling size. found RNA-binding protein nucleolin is associated importin β1 in axons. Perturbation association kinesins reduces its levels axons, concomitant reduction axonal levels. Strikingly, subcellular sequestration or enhances causes shift synthesis. Similar findings were obtained fibroblasts. Thus, both neurons cells.