Human iNKT Cells Promote Protective Inflammation by Inducing Oscillating Purinergic Signaling in Monocyte-Derived DCs

• DOI: 10.1016/j.celrep.2016.08.061
• Publication Date: 2016-09-20T14:32:20Z
• Authors: Xuequn Xu; Ginger M. Pocock; Akshat Sharma; Stephen L. Peery; J. Scott Fites; Laura Felley; Robert Zarnowski; Douglas Stewart; Erwin Berthier; Bruce S. Klein; Nathan M. Sherer; Jenny E. Gumperz
• Keywords: Inflammation; 0303 health sciences; QH301-705.5; Dendritic Cells; Monocytes; 3. Good health; Mice; 03 medical and health sciences; Animals; Humans; Natural Killer T-Cells; Receptors, Purinergic P2X7; Biology (General); Signal Transduction

Invariant natural killer T (iNKT) cells are innate T lymphocytes that promote host defense against a variety of microbial pathogens. Whether microbial ligands are required for their protective effects remains unclear. Here, we show that iNKT cells stimulate human-monocyte-derived dendritic cells (DCs) to produce inflammatory mediators in a manner that does not require the presence of microbial compounds. Interleukin 2 (IL-2)-exposed iNKT cells selectively induced repeated cytoplasmic Ca(2+) fluxes in DCs that were dependent on signaling by the P2X7 purinergic receptor and mediated by ATP released during iNKT-DC interactions. Exposure to iNKT cells led to DC cyclooxygenase 2 (PTGS2) gene transcription, and release of PGE2 that was associated with vascular permeabilization in vivo. Additionally, soluble factors were released that induced neutrophil recruitment and activation and enhanced control of Candida albicans. These results suggest that sterile interactions between iNKT cells and monocyte-derived DCs lead to the production of non-redundant inflammatory mediators that promote neutrophil responses.