Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells

• DOI: 10.1016/j.celrep.2016.08.093
• Publication Date: 2016-09-28T10:47:56Z
• Authors: Gérard Manière; Anna B. Ziegler; Flore Geillon; David E. Feathers...; Yael Grosjean
• Keywords: [SDV.BA] Life Sciences [q-bio]/Animal biology; heavy-chain; Amino Acid Transport Systems; Insulins; amino acid transporter; genetics [Drosophila Proteins]; cytology [Drosophila melanogaster]; Glutamate Dehydrogenase; Hemolymph; Insulin-Secreting Cells; metabolism [Drosophila melanogaster]; Drosophila;Drosophila insulin-like peptides;amino acid transporter;food;glutamate dehydrogenase;glycemia;growth;insulin-producing cells;minidiscs;starvation; Drosophila Proteins; Protein Isoforms; metabolism [Calcium]; genetics [Insulins]; genetics [Amino Acid Transport Systems]; Biology (General); minidiscs; 0303 health sciences; cytology [Larva]; pancreatic beta-cell; [SDV.BA]Life Sciences [q-bio]/Animal biology; glutamate dehydrogenase; Brain; metabolism [Hemolymph]; secretion; Drosophila melanogaster; Larva; Alimentation et Nutrition; [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]; Drosophila; Signal Transduction; glucose-transport; genetics [Glutamate Dehydrogenase]; genetics [Protein Isoforms]; QH301-705.5; growth; amino-acids; 610; metabolism [Drosophila Proteins]; 612; [SDV.BC]Life Sciences [q-bio]/Cellular Biology; metabolism [Larva]; insulin-producing cells; Article; metabolism [Amino Acid Transport Systems]; metabolism [Insulins]; 03 medical and health sciences; Leucine; Food and Nutrition; Animals; [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]; ddc:610; cytology [Insulin-Secreting Cells]; Mnd protein, Drosophila; [SDV.BC] Life Sciences [q-bio]/Cellular Biology; administration & dosage [Leucine]; metabolism [Protein Isoforms]; Ilp5 protein, Drosophila; cytology [Brain]; food; Neurosciences; starvation; metabolism [Insulin-Secreting Cells]; glutamate-dehydrogenase; l-leucine; glycemia; [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition; Gene Expression Regulation; metabolism [Brain]; metabolism [Glutamate Dehydrogenase]; Neurons and Cognition; metabolism [Leucine]; Calcium; Drosophila insulin-like peptides; [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition; fat-cells; metabolism

Dietary leucine has been suspected to play an important role in insulin release, a hormone that controls satiety and metabolism. The mechanism by which insulin-producing cells (IPCs) sense regulate secretion is still poorly understood. In Drosophila, insulin-like peptides (DILP2 DILP5) are produced brain IPCs released the hemolymph after ingestion. Using Ca(2+)-imaging ex vivo cultured larval brains, we demonstrate can directly extracellular levels via minidiscs (MND), transporter. MND knockdown abolished leucine-dependent changes, including loss of DILP2 DILP5 IPC bodies, consistent with idea necessary for DILP release. This, turn, leads strong increase sugar reduced growth. GDH also suggesting nutrient sensing coupled glutamate dehydrogenase pathway.