Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus

• DOI: 10.1016/j.celrep.2017.06.089
• Publication Date: 2017-07-25T18:45:51Z
• Authors: Maki Takagishi; Masato Sawada; Shinya Ohata; Naoya Asai; Atsushi Enomoto; Kunihiko Takahashi; Liang Weng; Kaori Ushida; Hosne Ara; Shigeyuki Matsui; Kozo Kaibuchi; Kazunobu Sawamoto; Masahide Takahashi
• Keywords: Ependymal Cell; Motile cilium; Cell polarity; Basal body

Motile cilia in ependymal cells, which line the cerebral ventricles, exhibit a coordinated beating motion that drives directional cerebrospinal fluid (CSF) flow and guides neuroblast migration. At apical cortex of these multi-ciliated asymmetric localization planar cell polarity (PCP) proteins is required for polarization microtubule dynamics, coordinates orientation. Daple disheveled-associating protein controls non-canonical Wnt signaling pathway motility. Here, we show Daple-deficient mice present hydrocephalus their lack regulates dynamics at anterior side turn orients cilial basal bodies flow. These results demonstrate an important role motile provide framework understanding mechanisms functions cells.