Pancreatic Islet Blood Flow Dynamics in Primates

• DOI: 10.1016/j.celrep.2017.07.039
• Publication Date: 2017-08-08T20:05:21Z
• Authors: Juan A. Diez; Rafael Arrojo e D...; Xiaofeng Zheng; Olga V. Stelmashenko; Minni Chua; Rayner Rodriguez-...; Masahiro Fukuda; Martin Köhler; Ingo Leibiger; Sai Bo Bo Tun; Yusuf Ali; George J. Augustine; Veluchamy A. Barathi; Per-Olof Berggren
• Keywords: Pancreatic Islets

Blood flow regulation in pancreatic islets is critical for function but poorly understood. Here, we establish an vivo imaging platform a non-human primate where transplanted autologously into the anterior chamber of eye are monitored non-invasively and longitudinally at single-cell resolution. Engrafted were vascularized innervated maintained cytoarchitecture situ pancreas. velocity engrafted was not affected by increasing blood glucose levels and/or GLP-1R agonist liraglutide. However, islet dynamic nature fluctuated various capillaries. This associated with vasoconstriction events resembling sphincter-like action, most likely regulated adrenergic signaling. These observations suggest mechanism that diverts to cell regions higher metabolic demand. The described technology applied may contribute better understanding human pathophysiology.