Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins

• DOI: 10.1016/j.celrep.2017.08.036
• Publication Date: 2017-09-05T16:15:34Z
• Authors: Rasmus Iversen; Omri Snir; Maria Stensland; José E. Kroll; Øyvind Steinsbø; Ilma R. Korponay-...; Knut E.A. Lundin; Gustavo A. de Souza; Ludvig M. Sollid
• Keywords: Immunoglobulin A; Lamina propria; J chain; Secretory component

Mucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study relationship between mucosal and systemic antibody responses, we took advantage celiac disease patient samples isolation gut PCs as well serum IgA IgG reactive with a gluten-derived peptide or autoantigen transglutaminase 2. Proteomic analysis revealed antigen-specific V-gene preferences, which matched those found in PCs. Further, PC CDR-H3 sequences were abundant but also detectable IgG. Our data indicate same B cell clones give rise to contribute pool. However, antibodies had molecular composition distinct from secreted gut, suggesting individual different populations.