TIM-1 Ubiquitination Mediates Dengue Virus Entry

• DOI: 10.1016/j.celrep.2018.04.013
• Publication Date: 2018-05-25T08:11:47Z
• Authors: Ophélie Dejarnac; Mohamed Lamine Ha...; Maxime Chazal; Margaux Versapuech; Julien Gaillard; Manuel Perera-Lecoin; Claudia Umana-Diaz; Lucie Bonnet-Madin; Xavier Carnec; Jean-Yves Tinevez; Constance Delaugerre; Olivier Schwartz; Philippe Roingeard; Nolwenn Jouvenet; Clarisse Berlioz-...; Laurent Meertens; Ali Amara
• Keywords: Internalization; Antibody-dependent enhancement; ESCRT

Dengue virus (DENV) is a major human pathogen causing millions of infections yearly. Despite intensive investigations, DENV receptor that directly participates in internalization has not yet been characterized. Here, we report the phosphatidylserine TIM-1 an authentic entry plays active role endocytosis. Genetic ablation strongly impaired infection. Total internal reflection fluorescence microscopy analyses live infected cells show mostly confined clathrin-coated pits and co-internalized with during viral entry. ubiquitinated at two lysine residues its cytoplasmic domain, this modification required for Furthermore, STAM-1, component ESCRT-0 complex involved intracellular trafficking cargos, interacts Overall, our results first bona fide identified DENV.