Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing

• DOI: 10.1016/j.celrep.2020.107656
• Publication Date: 2020-05-19T14:50:05Z
• Authors: Emily C. Freund; Anne L. Sapiro; Qin Li; Sandra Linder; James J. Moresco; John R. Yates; Jin Billy Li
• Keywords: ADAR; Inosine; Druggability

Adenosine-to-inosine RNA editing is catalyzed by adenosine deaminase acting on (ADAR) enzymes that deaminate to inosine. Although many sites are known, few trans regulators have been identified. We perform BioID followed mass spectrometry identify of ADAR1 and ADAR2 in HeLa M17 neuroblastoma cells. known novel ADAR-interacting proteins. Using ENCODE data, we validate characterize a subset the interactors as global or site-specific regulators. Our set includes all four members DZF-domain-containing family proteins: ILF3, ILF2, STRBP, ZFR. show these proteins interact with each ADAR modulate levels. find ILF3 broadly influential negative regulator editing. This work demonstrates broad roles binding play regulating levels, establishes group highly