Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo

• DOI: 10.1016/j.celrep.2020.107887
• Publication Date: 2020-07-14T14:38:11Z
• Authors: Hirofumi Aso; Shumpei Nagaoka; Eiryo Kawakami; Jumpei Ito; Saiful Islam; Benjy Jek Yang Tan; Shinji Nakaoka; Koichi Ashizaki; Katsuyuki Shiroguchi; Yutaka Suzuki; Yorifumi Satou; Yoshio Koyanagi; Kei Sato
• Keywords: Male; 0303 health sciences; QH301-705.5; Green Fluorescent Proteins; HIV; HIV Infections; Genomics; 3. Good health; Mice; 03 medical and health sciences; HEK293 Cells; HIV-1; Animals; Humans; humanized mouse; Female; Biology (General); Transcriptome; transcriptome; genome; multiomics

For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity HIV-1-infected cells in vivo. To reveal multiple characteristics HIV-1-producing vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We perform multiomics experiments using recently developed technology identify cells. Genome-wide integration-site analysis reveals that productive infection tends occur viral integration into transcriptionally active genomic regions. Bulk transcriptome high level mRNA transcribed Moreover, single-cell shows cells, including CXCL13high subpopulation low expression interferon-stimulated genes, which can contribute efficient spread Our findings describe could provide clues for development an cure.