Large, Stable Spikes Exhibit Differential Broadening in Excitatory and Inhibitory Neocortical Boutons

• DOI: 10.1016/j.celrep.2020.108612
• Publication Date: 2021-01-14T06:08:07Z
• Authors: Andreas Ritzau-Jost; Timur Tsintsadze; Martin Krueger; Jonas Ader; Ingo Bechmann; Jens Eilers; Boris Barbour; Stephen M. Smith; Stefan Hallermann
• Keywords: Neocortex

Presynaptic action potential spikes control neurotransmitter release and thus interneuronal communication. However, the properties dynamics of presynaptic in neocortex remain enigmatic because boutons are small direct patch-clamp recordings have not been performed. Here, we report from neocortical pyramidal neurons interneurons. Our data reveal rapid large potentials layer 5 fast-spiking interneurons reliably propagating into axon collaterals. For in-depth analyses, establish mature cultured as models for excitatory boutons, demonstrating that spike amplitude is unaffected by potassium channels, homeostatic long-term plasticity, high-frequency firing. In contrast to stable amplitude, profoundly broaden during firing neurons, but Thus, our demonstrate fundamental differences between inhibitory neocortex.