Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4+ T cell subsets in vivo

• DOI: 10.1016/j.celrep.2021.109038
• Publication Date: 2021-04-27T16:21:49Z
• Authors: Guorui Xie; Xiaoyu Luo; Tongcui Ma; Julie Frouard; Jason Neidleman; Rebecca Hoh; Steven G. Deeks; Warner C. Greene; Nadia R. Roan
• Keywords: Mass cytometry

Relatively little is known about features of T cells targeted by HIV in vivo. By applying bioinformatics analysis to mass cytometry (CyTOF)-phenotyped specimens from individuals with viremia and in-vitro-infected uninfected donors, we provide an atlas the phenotypes vivo vitro HIV-susceptible cells. helper 17 (Th17) α4β1+ are preferentially vivo, whereas effector memory (Tem), transitional (Ttm), Th1, Th1/Th17 subsets vitro. Multiple proteins—including chemokine cytokine receptors—are remodeled these changes mostly recapitulated remodels a follicular (Tfh) phenotype. Using clustering, uncover subset CD29-expressing, Tem-like that highly susceptible infection experimentally confirm susceptibility. These studies in-depth look at demonstrate some—but not all—HIV-susceptible identified effectively model