Axon-enriched lincRNA ALAE is required for axon elongation via regulation of local mRNA translation

• DOI: 10.1016/j.celrep.2021.109053
• Publication Date: 2021-05-04T14:28:03Z
• Authors: Manyi Wei; Jiansong Huang; Guo-Wei Li; Bowen Jiang; Hong Cheng; Xiaoyan Liu; Xingyu Jiang; Xu Zhang; Li Yang; Lan Bao; Bin Wang
• Keywords: Dorsal root ganglion

Long intergenic noncoding RNAs (lincRNAs) are critical regulators involved in diverse biological processes. However, the roles and related mechanisms of lincRNAs axon development largely unknown. Here we report an axon-enriched lincRNA regulating elongation, referred to as ALAE. Profiling highly expressed detected abundant enriched ALAE axons dorsal root ganglion (DRG) neurons, where it locally promoted elongation. Mechanically, directly interacted with KH3–4 domains KH-type splicing regulatory protein (KHSRP) impeded its association growth-associated 43 (Gap43) mRNA. Knockdown reduced but not mRNA level GAP43 DRG neurons. Furthermore, local disruption interaction between KHSRP significantly inhibited Gap43 translation, impairing This study implies crucial spatiotemporal regulation translation during development.