Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes

• DOI: 10.1016/j.celrep.2021.109100
• Publication Date: 2021-05-11T15:53:24Z
• Authors: Eva Riechert; Vivien Kmietczyk; Frank Stein; Thomas Schwarzl; Thileepan Sekaran; Lonny Jürgensen; Verena Kamuf-Schenk; Eshita Varma; Christoph Hofmann; Mandy Rettel; Kira Gür; Julie Ölschläger; Friederike Kühl; Judit Martin; Marta Ramirez-Ped...; Mercedes Fernandez; Shirin Doroudgar; Raúl Méndez; Hugo A. Katus; Matthias W. Hentze; Mirko Völkers
• Keywords: Interactome

RNA-binding proteins (RBPs) control critical aspects of cardiomyocyte function, but the repertoire active RBPs in cardiomyocytes during growth response is largely unknown. We define healthy and diseased at a system-wide level by RNA interactome capture. This identifies 67 cardiomyocyte-specific RBPs, including several contractile proteins. Furthermore, we identify cytoplasmic polyadenylation element-binding protein 4 (Cpeb4) as dynamic RBP, regulating cardiac both vitro vivo. mRNAs bound to regulated Cpeb4 cardiomyocytes. regulates remodeling differential expression transcription factors. Among target mRNAs, two zinc finger factors (Zeb1 Zbtb20) are discovered. show that these depletion increases their expression. Thus, emerges regulator function binding specific pathological stimulation.