A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression

• DOI: 10.1016/j.celrep.2021.109499
• Publication Date: 2021-08-03T16:20:01Z
• Authors: Anna Brachet; Argentina Lario; Alba Fernández-Ro...; Frank F. Heisler; Yolanda Gutiérrez; Clara Lobo; Matthias Kneussel; José A. Esteban
• Keywords: Long-term depression; Kinesin; Internalization; Silent synapse

The synaptic removal of AMPA-type glutamate receptors (AMPARs) is a core mechanism for hippocampal long-term depression (LTD). In this study, we address the role microtubule-dependent transport AMPARs as driver vesicular trafficking and sorting during LTD. Here, show that kinesin-1 motor KIF5A/C strictly required LTD expression in CA3-to-CA1 synapses. Specifically, find KIF5 an efficient internalization after NMDA receptor activation. We KIF5/AMPAR complex assembled activity-dependent manner associates with microsomal membranes upon induction. This interaction facilitated by adaptor protrudin, which also expression. propose protrudin links KIF5-dependent to endosomal sorting, preventing AMPAR recycling synapses Therefore, work identifies molecular protein executes