Cyclic-di-GMP signaling controls metabolic activity in Pseudomonas aeruginosa

• DOI: 10.1016/j.celrep.2022.111515
• Publication Date: 2022-10-18T15:33:08Z
• Authors: Mads Lichtenberg; Kasper Nørskov Kragh; Blaine Fritz; Julius B. Kirkegaard; Tim Tolker-Nielsen; Thomas Bjarnsholt
• Keywords: Metabolic pathway; Pseudomonadales

Bacteria in biofilms are embedded extracellular matrix and display low metabolic activity, partly due to insufficient diffusive exchange of substrate. The activity both contribute the high antibiotic tolerance-the hallmark biofilm bacteria. second messenger molecule, c-di-GMP, regulates development Pseudomonas aeruginosa, where internal levels lead formation associated with planktonic Using a microcalorimetric approach, we show that c-di-GMP signaling is major determinant P. culture two models. content bacteria forces them rapidly spend large amount energy on production exopolysaccharides, resulting subsequent state. This suggests state mature biofilms, some extent, consequence c-di-GMP-regulated survival strategy.