Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis

• DOI: 10.1016/j.celrep.2024.113716
• Publication Date: 2024-02-26T12:44:38Z
• Authors: Jia Yan; Ling Zhou; Mengya Liu; Honglan Zhu; Xin Zhang; E. Cai; Xueqiang Xu; Tinghan Chen; Hongyan Cheng; Jun’e Liu; Shang Wang; Lin Dai; Xiaohong Chang; Fuchou Tang
• Keywords: 0303 health sciences; 03 medical and health sciences; QH301-705.5; CP: Developmental biology; Biology (General)

Ovarian endometriosis is characterized by the growth of endometrial tissue within ovary, causing infertility and chronic pain. However, its pathophysiology remains unclear. Utilizing high-precision single-cell RNA sequencing, we profile normal, eutopic, ectopic endometrium from 34 individuals across proliferative secretory phases. We observe an increased proportion ciliated cells in both eutopic endometrium, a diminished expression estrogen sulfotransferase, which likely confers apoptosis resistance. After translocating to lesions, epithelium upregulates nicotinamide N-methyltransferase that inhibits promoting deacetylation subsequent nuclear exclusion transcription factor forkhead box protein O1, thereby leading downregulation apoptotic gene BIM. Moreover, epithelial lesions elevate HLA class II complex expression, stimulates CD4