Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection

• DOI: 10.1016/j.chom.2023.11.020
• Publication Date: 2023-12-15T16:37:41Z
• Authors: Shumei Wang; Baoye He; Huaitong Wu; Qiang Cai; Obed Ramírez-Sánchez; Cei Abreu-Goodger; Paul R.J. Birch; Hailing Jin
• Keywords: Pathogenic fungus

Cross-kingdom small RNA trafficking between hosts and microbes modulates gene expression in the interacting partners during infection. However, whether other RNAs are also transferred is unclear. Here, we discover that host plant Arabidopsis thaliana delivers mRNAs via extracellular vesicles (EVs) into fungal pathogen Botrytis cinerea. A fluorescent aptamer reporter Broccoli system reveals EVs recipient cells. Using translating ribosome affinity purification profiling polysome analysis, observe delivered translated Ectopic of two B. cinerea shows their proteins detrimental to knockout mutants genes corresponding these more susceptible. Thus, plants have a strategy reduce infection by transporting from pathogenic fungi compromise infection, providing knowledge helps combat crop diseases.