Development, characterization and application of 3D printed adsorbents for in situ recovery of taxadiene from microbial cultivations

• DOI: 10.1016/j.chroma.2024.464815
• Publication Date: 2024-03-19T02:21:21Z
• Authors: Giuseppe Rafael G...; M. Sulaiman Sarwar; Leonardo Rios-Solis; Simone Dimartino
• Keywords: Paclitaxel; Downstream processing; Terpenes; Taxadiene; Printing, Three-Dimensional; In situ product recovery; Methacrylates; 3D printing; Saccharomyces cerevisiae; Diterpenes; Alkenes; Solid adsorption

Microbial cell factories are an attractive alternative to produce high-value natural products using sustainable processes. However, product recovery is one of the main challenges reduce production cost and make these technologies economically interesting. In this work, new resins were formulated 3D print hydrophobic adsorbents for biologics from microbial cultivations. Benzyl methacrylate (BEMA) Butyl (BUMA) selected as functional monomers suitable adsorption compounds. Pore morphology was tailored through inclusion pore forming agents (porogens) in resin. Different porogens porogen concentrations evaluated resulting materials with different porous networks. Sudan 1 anticancer drug paclitaxel employed model compounds test performance terpene molecules onto developed printed materials. The material greatest capacity obtained BEMA monomer 40% (v/v) (BEMA40). BEMA40 recover taxadiene small-scale (5 mL) Saccharomyces cerevisiae cultivations tested compared commercial Diaion HP-20 beads. Taxadiene titres on (46 ± 2 mg/L) (54 4 comparable, no detected cells cell-free media, suggesting near 100% partition adsorbents. Compared beads, can be customized adjustments resin formulation, well adaptable diverse bioreactor types, do not clog sampling ports columns easier handle during post processing. results work demonstrate potential printing fabricate interaction adsorbent their application biological products.