Molecular diagnosis appended by histopathological signature delineates the white spot syndrome virus (WSSV) infection in penaeid shrimps

• DOI: 10.1016/j.cirep.2024.200138
• Publication Date: 2024-02-13T06:04:57Z
• Authors: Md. Juwel Hasan; Shirin Sultana; Md. Nasir Khan; H.M. Rakibul Islam; Mohammad Nazrul I...
• Keywords: White spot syndrome; White (mutation); Penaeidae

White spot disease (WSD) occurred by the highly devastating white syndrome virus (WSSV) poses a serious impediment toward global shrimp production. We investigated molecular diagnosis and natural infection with WSSV in cultured tiger (Penaeus monodon) inspected histopathology of WSD. The incidence was recorded six small-scale farms situated two districts Bangladesh, namely Bagerhat Satkhira. One- two-step nested polymerase chain reaction models were applied to check presence DNA samples. By one-step PCR, 25 36 samples WSSV-positive, while remaining 11 found be WSSV-negative. After negative underwent PCR process, only three detected negative. In Satkhira districts, prevalence rates 83.33% 55.56%, respectively, as confirmed PCR; following remained unchanged for but increased 100% sourced One-step less effective than diagnosing viral infection; instantly evident that overall rate rose from 69.44% 91.67% when switching PCR. different experiment, histological analyses PCR-confirmed WSSV-positive specimens revealed degenerated tubule lumen hepatopancreas necrosis, infected muscle tissues showed fiber fragmentation separation. Based on technique histopathological evidence infection, study demonstrated occurrence farms. combined practices diagnostic tools information will useful understanding managing its prevention production systems.