A soybean NAC homolog contributes to resistance to Phytophthora sojae mediated by dirigent proteins

• DOI: 10.1016/j.cj.2021.08.009
• Publication Date: 2021-10-25T19:20:50Z
• Authors: Guolong Yu; Jianan Zou; Jinhui Wang; Rongsheng Zhu; Zhaoming Qi; Hongwei Jiang; Zhenbang Hu; Mingliang Yang; Ying Zhao; Xiaoxia Wu; Chunyan Liu; Candong Li; Xue Yang; Zhendong Zhu; Qingshan Chen; Yongfu Fu; Dawei Xin
• Keywords: Phytophthora sojae; Immunoprecipitation; Transcription

Phytophthora sojae infection severely impairs soybean production. We previously identified a dirigent protein, GmDRR1 (Glycine max Disease Resistant Response 1), that increases resistance to P. sojae. However, the molecular basis of function remained largely uncharacterized. In present study, analysis GmDRR1-RNAi, GmDRR1-overexpressing, and CRISPR/Cas9-derived Gmdrr1 mutant lines revealed expression significantly restricted growth. Combining co-immunoprecipitation with liquid chromatography–tandem mass spectrometry GmDRR1-interacting GmDRR2, which is homologous GmDRR1. An E-coniferyl alcohol coupling assay indicated promotes synthesis (+)-pinoresinol, helps protect plants from The GmNAC1 (Glyma.05G025500) transcription factor bound promoter both in vitro vivo upregulate expression. Soybean was increased by overexpression GmNAC1. Our findings suggest novel signaling pathway involving NAC mediates Specifically, directly induces increase