Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24

• DOI: 10.1016/j.cj.2021.12.003
• Publication Date: 2022-01-11T18:44:07Z
• Authors: Qian Zhou; Bingzhi Jiang; Yanbo Cheng; Qibin Ma; Qiuju Xia; Ze Jiang; Zhandong Cai; Hai Nian
• Keywords: Genetic linkage

Powdery mildew (PM), caused by the fungus Microsphaera diffusa, causes severe yield losses in soybean [Glycine max (L.) Merr.] under suitable environmental conditions. Identifying resistance genes and developing resistant cultivars may prevent PM damage. In this study, analysis of F1, F2, F8:11 recombinant inbred line (RIL) populations derived from cross between Zhonghuang 24 (ZH24) Huaxia 3 (HX3) indicated that adult-plant (APR) to powdery cultivar (cv.) ZH24 was controlled a single dominant locus. A high-density genetic linkage map RIL population used for fine mapping. The APR locus mapped 281-kb genomic region on chromosome 16. Using 283 susceptible plants another F2 population, candidate fine-mapped 32.8-kb interval flanked markers InDel14 Gm16_428. harbored five genes, including four disease (R)-like according Williams 82.a2.v1 reference genome. Quantitative real-time PCR assays revealed expression levels Glyma.16g214300 Glyma.16g214500 were changed M. diffusa infection might be involved defense. Rmd_B13 showed all-stage (ASR) cv. B13. An allelism test segregating × B13 suggested Rmd_ZH24 ASR allelic or tightly linked. These results provide marker-assisted selection breeding programs.