Elite, transformable haploid inducers in maize

• DOI: 10.1016/j.cj.2023.10.016
• Publication Date: 2023-12-21T02:25:31Z
• Authors: Brent Delzer; Dawei Liang; David Szwerdszarf; Isadora Rodriguez; Gonzalo Mardones; Sivamani Elumalai; Francine Johnson; Samson Nalapalli; Rachel Egger; Erin Burch; Kerry Meier; Juan Wei; Xiujuan Zhang; Huaping Gui; Huaibing Jin; Huan Guo; Kun Yu; Yubo Liu; Becky Breitinger; Ana Poets; Jason Nichols; Wan Shi; David Skibbe; Qiudeng Que; Timothy Kelliher
• Keywords: Inducer; Elite

The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly. Two technologies that are disrupting traditional processes doubled haploid (DH) genome editing (GE). Recently, these techniques were combined a GE trait delivery system called HI-Edit (Haploid Inducer-Edit) [1]. In HI-Edit, the pollen inducer line reprogrammed to deliver traits any variety, obviating recurrent selection. For operate at scale, an efficient transformable HI needed, but most maize varieties recalcitrant transformation, inducers especially difficult transform given their aberrant reproductive behaviors. Leveraging marker assisted selection three-tiered testing scheme, we report development new Iodent Stiff Stalk germplasm transformable, have high induction rates, exhibit robust, genetically-dominant anthocyanin native may be used for rapid identification. We show transformation elite "HI-Edit" lines enhanced using BABYBOOM WUSCHEL morphogenetic factors. Finally, evaluate performance one against non-Stiff testers. strategy results this study should facilitate commercially scalable systems in diverse crops.