CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

• DOI: 10.1016/j.cj.2024.07.007
• Publication Date: 2024-07-31T02:29:37Z
• Authors: Le Gao; Lijun Xie; Yanmin Xiao; Xinge Cheng; Ruosi Pu; Ziheng Zhang; Yu Liu; Shaopei Gao; Zilong Zhang; Haoran Qu; Haijian Zhi; Kai Li
• Keywords: Soybean mosaic virus; Germ plasm

Soybean mosaic virus (SMV), an RNA virus, is the most common and destructive pathogenic in soybean fields. The newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses; hence, this study aimed engineer SMV using system. Specifically, multiple sgRNAs were designed target positive- and/or negative-sense strands of HC-Pro gene. Subsequently, corresponding CRISPR/CasRx vectors constructed transformed into soybeans. After inoculation with SMV, 39.02%, 35.77%, 18.70% T1 plants confirmed be highly resistant (HR), (R), mildly (MR) respectively, whereas only 6.50% identified as susceptible (S). Additionally, qRT-PCR DAS-ELISA showed that, both at 15 30 d post-inoculation (dpi), accumulation significantly decreased or was even undetectable HR R plants, followed by MR S plants. expression level CasRx gene varied almost all different level, dpi. Furthermore, when evaluated T2 generation, results similar those recorded generation. These findings provide new insights application improvement offer promising alternative breeding biotic stress that will contribute development SMV-immune germplasm accelerate progress towards greater crop productivity.