Microscale dynamics of electrophysiological markers of epilepsy

• DOI: 10.1016/j.clinph.2021.06.024
• Publication Date: 2021-08-02T15:44:35Z
• Authors: Jimmy C. Yang; Angelique C. Paulk; Pariya Salami; Sang Heon Lee; Mehran Ganji; Daniel J. Soper; Daniel Cleary; Mirela Simon; Douglas Maus; Jong Woo Lee; Brian V. Nahed; Pamela S. Jones; Daniel P. Cahill; Garth Rees Cosgrove; Catherine J. Chu; Ziv Williams; Eric Halgren; Shadi Dayeh; Sydney S. Cash
• Keywords: Microscale chemistry; Electrocorticography; Local field potential

AbstractObjectiveInterictal discharges (IIDs) and high frequency oscillations (HFOs) are neurophysiologic biomarkers of epilepsy. In this study, we use custom poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) microelectrodes to better understand their microscale dynamics.MethodsElectrodes with spatial resolution down to 50µm were used to record intraoperatively in 30 subjects. For IIDs, putative spatiotemporal paths were generated by peak-tracking, followed by clustering. For HFOs, repeating patterns were elucidated by clustering similar time windows. Fast events, consistent with multi-unit activity (MUA), were covaried with either IIDs or HFOs.ResultsIIDs seen across the entire array were detected in 93% of subjects. Local IIDs, observed across <50% of the array, were seen in 53% of subjects. IIDs appeared to travel across the array in specific paths, and HFOs appeared in similar repeated spatial patterns. Finally, microseizure events were identified spanning 50-100µm. HFOs covaried with MUA, but not with IIDs.ConclusionsOverall, these data suggest micro-domains of irritable cortex that form part of an underlying pathologic architecture that contributes to the seizure network.SignificanceMicroelectrodes in cases of human epilepsy can reveal dynamics that are not seen by conventional electrocorticography and point to new possibilities for their use in the diagnosis and treatment of epilepsy.HighlightsPEDOT:PSS microelectrodes with at least 50µm spatial resolution uniquely reveal spatiotemporal patterns of markers of epilepsyHigh spatiotemporal resolution allows interictal discharges to be tracked and reveal cortical domains involved in microseizuresHigh frequency oscillations detected by microelectrodes demonstrate localized clustering on the cortical surface