Ingestion of orange juice prevents hyperglycemia and increases plasma miR-375 expression

• DOI: 10.1016/j.clnesp.2021.12.003
• Publication Date: 2021-12-04T02:43:26Z
• Authors: Bruna J. Quintanilha; Daniela F.S. Chaves; Elisa Brasili; Telma A.F. Corrêa; Vinícius Cooper C...; Frederico Moraes ...; Inar A. Castro; Neuza M.A. Hassim...; Marcelo M. Rogero; Franco M. Lajolo
• Keywords: 2. Zero hunger; Eating; MicroRNAs; 0303 health sciences; 03 medical and health sciences; Cross-Over Studies; Diabetes Mellitus, Type 2; Hyperglycemia; Humans; Citrus sinensis; 3. Good health

The intake of high-fat, high-carbohydrate (HFHC) meals is associated with an increased risk of type 2 diabetes. There is evidence that the association of orange juice to a HFHC meal can modulate the expression of microRNAs (miRNAs) linked to pancreatic β-cell function such as miR-375. We evaluated the effect of a commercial orange juice intake with HFHC meal on plasma miRNAs expression in twelve healthy subjects in a crossover design study.Subjects ingested water, orange juice, or an isocaloric beverage along with a 1037 kcal HFHC meal. Blood glucose and miRNAs were evaluated at baseline and 1, 3, and 5 h after the intake.The area under the curve (AUC) for glycemia after ingestion of HFHC + orange juice did not differ from ingestion of HPHC + glucose or HFHC + water. However, the AUC was higher in HFHC meal + glucose compared to HFHC meal + water (p = 0.034). Glucose and insulin concentrations were significantly higher in HFHC meal + glucose group after 1 h, when compared with other groups and times (p < 0.001). There was an increase in plasma miR-375 expression after 3 h of ingestion of HFHC + orange juice versus water (p = 0.026), and a decrease in plasma miR-205-5p expression after HFHC meal + glucose versus water (p = 0.023).A single HFHC meal + orange juice modulated plasma miR-375 expression, which is a biomarker of pancreatic β-cell function, and contributed to preventing hyperglycemia.