Whole genome sequencing of Shigella sonnei through PulseNet Latin America and Caribbean: advancing global surveillance of foodborne illnesses

• DOI: 10.1016/j.cmi.2017.03.021
• Publication Date: 2017-04-04T13:40:35Z
• Authors: K.S. Baker; J. Campos; M. Pichel; A. Della Gaspera; F. Duarte-Martínez; E. Campos-Chacón; H.M. Bolaños-Acuña; C. Guzmán-Verri; A.E. Mather; S. Diaz Velasco; M.L. Zamudio Rojas; J.L. Forbester; T.R. Connor; K.H. Keddy; A.M. Smith; E.A. López de Del...; G. Angiolillo; N. Cuaical; J. Fernández; C. Aguayo; M. Morales Aguilar; C. Valenzuela; A.J. Morales Medrano; A. Sirok; N. Weiler Gustafson; P.L. Diaz Guevara; L.A. Montaño; E. Perez; N.R. Thomson
• Keywords: Shigella sonnei

Shigella sonnei is a globally important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed demonstrate possibility advantages of transitioning whole genome sequencing (WGS) for within existing networks across continent where S. endemic.We applied WGS representative archive isolates (n = 323) from nine countries generate regional phylogenomic reference put this global context. used contextualise 16 three Argentinian outbreaks, using locally generated sequence data. Assembled sequences were predict antimicrobial resistance (AMR) phenotypes identify AMR determinants.S. clustered five American sublineages phylogeny, with many (46%, 149 belonging previously undescribed sublineages. Predicted multidrug was (77%, 249 323), clinically relevant differences found among The overview showed that outbreak belonged distinct had different epidemiologic origins.Latin contains novel genetic diversity on scale commonly exhibits resistance. Retrospective passive has utility informing treatment, identifying regionally epidemic providing framework interpretation prospective, sequenced outbreaks.