Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles

• DOI: 10.1016/j.colsurfb.2017.09.044
• Publication Date: 2017-09-20T21:16:20Z
• Authors: Bożena Tyliszczak; Anna Drabczyk; Sonia Kudłacik-Kr...; Katarzyna Bialik-Wąs; Regina Kijkowska; Agnieszka Sobczak...
• Keywords: 0301 basic medicine; Chitosan; Staphylococcus aureus; Microbial Viability; Silver; Cell Survival; Metal Nanoparticles; Epithelial Cells; Hydrogels; Dermis; Body Fluids; Cell Line; 3. Good health; 03 medical and health sciences; Species Specificity; Biomimetic Materials; Enterococcus faecalis; Humans

Chitosan based hydrogels are commonly applied in various fields of medicine and pharmacy. Modification of hydrogel polymers using nanosilver particles may result in formation of materials with enhanced antibacterial properties. In this article synthesis of hydrogel materials based on chitosan and modified with silver nanoparticles is presented. First, preparation and characterization of silver nanoparticles using UV-vis spectroscopy has been shown. Hydrogels modified with nanosilver particles were subjected to the measurements of swelling ability and in vitro tests in distilled water and Simulated Body Fluid (SBF), respectively. Additionally, evaluation of antibacterial properties against Staphylococcus aureus and Enterococcus faecalis as well as results of cytotoxicity of hydrogel materials modified with silver nanoparticles conducted by means of XTT and MTT assays using dermis cells BJ (CRL-2522TM) have been presented.