Proteomic analyses revealed the antibacterial mechanism of Aronia melanocarpa isolated anthocyanins against Escherichia coli O157: H7

• DOI: 10.1016/j.crfs.2022.09.017
• Publication Date: 2022-09-15T09:40:07Z
• Authors: Haotian Deng; Yanwen Kong; Jinyan Zhu; Xinyao Jiao; Yuqi Tong; Meizhi Wan; Yang Zhao; Sixu Lin; Yan Ma; Xianjun Meng
• Keywords: Tandem mass tag

Aronia melanocarpa anthocyanins (AMAs), as a natural plant extract, can control pathogens and are attracting increasing attention. However, at the proteomic level, antibacterial mechanism of AMAs against Escherichia coli O157: H7 remains unclear. In this study, tandem mass tag (TMT) quantitative proteomics was used to elucidate potential mechanisms E. cells. Ultrastructural observation reactive oxygen species (ROS) levels detection showed that destroyed integrity cell membrane, led aggregation contents caused increase intracellular ROS level. TMT-based analysis suggested enter cells through mechanosensitive channels inhibit activity heat shock proteins; disturb metabolism carbohydrates, amino acids lipids in The results study provide reference for further development plant-derived antimicrobial agents.