Rhein against Staphylococcus xylosus by interfering with respiratory metabolism and inducing oxidative stress

• DOI: 10.1016/j.crfs.2024.100718
• Publication Date: 2024-03-16T16:54:09Z
• Authors: Yuyang Li; Weiwei Chen; Jinxin Ma; Guoying Huang; Guangquan Li; Qiumei He; Xiangyu Kong; Ling Tang; Jinqing Chen; Wenyou Ding; Zhongbin Zhang; Wenya Ding
• Keywords: Staphylococcus xylosus

Currently, dairy mastitis caused by Staphylococcus xylosus poses a serious challenge for farming. In this study, we explored the role and mechanism of rhein against S. with hope providing new research ideas to solve in cows ensure source safety products. Through vitro antimicrobial studies, found that minimum inhibitory concentration (MIC) was 64 μg/mL, it significantly interfered formation biofilm at sub-MIC. experiments on mice, alleviated inflammation mammary tissue, reduced levels TNF-α IL-6, decreased number xylosus. To explore anti-S. rhein, identified relevant proteins involved carbon metabolism (Glycolysis/gluconeogenesis, TCA cycle, Fatty acid degradation) through proteomics. Additionally, associated respiratory chain, oxidative stress (proteins antioxidant DNA repair), nitrate respiration were also be upregulated. Thus, may act as an antibacterial agent interfering inducing production ROS, high which alter permeability bacterial cell membranes cause damage them. We measured concentrations extracellular β-galactosidase nucleic acids. SEM observation morphology showed elevated membrane membrane. Finally, RT-PCR mRNAs key cycle (odhA, mqo) (nreB, nreC, narG) up-regulated, consistent proteomic results. conclusion, has good effects vivo, energy metabolism, ROS production, causing damage, one important mechanisms its activity.