High-resolution imaging of protein secretion at the single-cell level using plasmon-enhanced FluoroDOT assay

• DOI: 10.1016/j.crmeth.2022.100267
• Publication Date: 2022-08-05T14:31:41Z
• Authors: Anushree Seth; Ekansh Mittal; Jingyi Luan; Samhitha Kolla; Monty B. Mazer; Hemant Joshi; Rohit Gupta; Priya Rathi; Zheyu Wang; Jeremiah J. Morri...; Joel D. Ernst; Cynthia Portal-Ce...; Sharon Celeste Mo...; Jennifer A. Philips; Srikanth Singamaneni
• Keywords: Live cell imaging; Secretory protein

Secreted proteins mediate essential physiological processes. With conventional assays, it is challenging to map the spatial distribution of secreted by single cells, study cell-to-cell heterogeneity in secretion, or detect low abundance incipient secretion. Here, we introduce "FluoroDOT assay," which uses an ultrabright nanoparticle plasmonic-fluor that enables high-resolution imaging protein We find plasmonic-fluors are 16,000-fold brighter, with nearly 30-fold higher signal-to-noise compared fluorescence labels. demonstrate different cytokines single-plexed and spectrally multiplexed FluoroDOT assay revealed cellular secretion multiple simultaneously. Using diverse biochemical stimuli, including