Noninvasive focal transgene delivery with viral neuronal tracers in the marmoset monkey

• DOI: 10.1016/j.crmeth.2024.100709
• Publication Date: 2024-02-14T15:44:11Z
• Authors: T. Vincenza Parks; Diego Szczupak; Sang-Ho Choi; David J. Schaeffer
• Keywords: 0301 basic medicine; 03 medical and health sciences; Report

We establish a reliable method for selectively delivering adeno-associated viral vectors (AAVs) across the blood-brain barrier (BBB) in marmoset without need neurosurgical injection. focally perturbed BBB (∼1 × 2 mm) area 8aD of frontal cortex four adult monkeys using low-intensity transcranial focused ultrasound aided by microbubbles. Within an hour opening BBB, either AAV2 or AAV9 was delivered systemically via tail-vein In all marmosets, fluorescence-encoded neurons were observed at site perturbation, with showing sparse distribution transduced when compared to AAV9. The results are direct intracortical injections anterograde tracers into and similar (albeit sparser) long-range connectivity observed. With evidence specific region as well long-distance tracing, we framework focal noninvasive transgene delivery brain.