Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals

• DOI: 10.1016/j.cub.2018.02.032
• Publication Date: 2018-03-15T15:47:21Z
• Authors: Eugene Jennifer Jin; Ferdi Ridvan Kiral; Mehmet Neset Ozel; Lara Sophie Burch...; Marc Osterland; Daniel Epstein; Heike Wolfenberg; Steffen Prohaska; Peter Robin Hiesi...
• Keywords: Axon terminal

Neurons are highly polarized cells that require continuous turnover of membrane proteins at axon terminals to develop, function, and survive. Yet, it is still unclear whether protein degradation requires transport back the cell body or also occurs locally terminal, where live observation sorting has remained a challenge. Here, we report direct two cargo-specific mechanisms based on live-imaging approach in intact Drosophila brains. We show different acidification-sensing cargo probes sorted into distinct classes degradative "hub" compartments for synaptic vesicle plasma terminals. Sorting cargoes separate hubs molecularly distinct. Local terminal is, surprisingly, Rab7 independent, whereas dependent. The cathepsin-like protease CP1 specific hubs, its delivery SNARE neuronal synaptobrevin. Cargo separation only mixed. These data least local, pathways sort specifically can occur both en route body.