Decoupling Yeast Cell Division and Stress Defense Implicates mRNA Repression in Translational Reallocation during Stress

• DOI: 10.1016/j.cub.2018.06.044
• Publication Date: 2018-08-02T14:43:14Z
• Authors: Yi-Hsuan Ho; Evgenia Shishkova; James Hose; Joshua J. Coon; Audrey P. Gasch
• Keywords: Decoupling (probability)

Stress tolerance and rapid growth are often competing interests in cells. Upon severe environmental stress, many organisms activate defense systems concurrent with arrest. There has been debate as to whether aspects of the stress-activated transcriptome regulated by stress or an indirect byproduct reduced proliferation. For example, stressed Saccharomyces cerevisiae cells mount a common gene expression program called response (ESR) [1Gasch A.P. Spellman P.T. Kao C.M. Carmel-Harel O. Eisen M.B. Storz G. Botstein D. Brown P.O. Genomic programs yeast changes.Mol. Biol. Cell. 2000; 11: 4241-4257Crossref PubMed Scopus (3730) Google Scholar] comprised ∼300 induced (iESR) transcripts involved ∼600 (rESR) mRNAs encoding ribosomal proteins (RPs) ribosome biogenesis factors (RiBi) important for division. Because ESR activation also correlates rate nutrient-restricted chemostats prolonged G1 slow-growing mutants, alternate proposal is that simply consequence division [2Castrillo J.I. Zeef L.A. Hoyle D.C. Zhang N. Hayes A. Gardner Cornell M.J. Petty J. Hakes L. Wardleworth et al.Growth control eukaryote cell: biology study yeast.J. 2007; 6: 4Crossref (207) Scholar, 3Brauer Huttenhower C. Airoldi E.M. Rosenstein R. Matese J.C. Gresham Boer V.M. Troyanskaya O.G. Coordination rate, cell cycle, response, metabolic activity yeast.Mol. 2008; 19: 352-367Crossref (399) 4Regenberg B. Grotkjaer T. Winther Fausbøll Akesson M. Bro Hansen L.K. Brunak S. Nielsen Growth-rate genes have profound impact on interpretation profiling cerevisiae.Genome 2006; 7: R107Crossref (189) 5O'Duibhir E. Lijnzaad P. Benschop J.J. Lenstra T.L. van Leenen Groot Koerkamp Margaritis Brok M.O. Kemmeren Holstege F.C. Cell cycle population effects perturbation studies.Mol. Syst. 2014; 10: 732Crossref (68) Scholar]. A major challenge past studies did not separate arrest defense; thus, true responsiveness ESR—and purpose stress-dependent rESR repression particular—remains unclear. Here, we decoupled from following transcriptome, proteome, polysome changes arrested responding acute stress. We show cannot be explained cell-cycle phase during acclimation. Instead, failure repress reduces association transcripts, delaying production their proteins. Our results suggest alleviate competition translation removing ribosomes translating pool, directing translational capacity toward accelerate protein production.