LCO Receptors Involved in Arbuscular Mycorrhiza Are Functional for Rhizobia Perception in Legumes

• DOI: 10.1016/j.cub.2019.11.038
• Publication Date: 2019-12-05T15:56:28Z
• Authors: Ariane Girardin; Tongming Wang; Yi Ding; Jean Keller; Luis Buendia; Mégane Gaston; Camille Ribeyre; Virginie Gasciolli; Marie-Christine A...; Tatiana Vernié; Abdelhafid Bendah...; Martina Katharina...; Martin Parniske; Patrice Morel; Michiel Vandenbus...; Martine Schorderet; Didier Reinhardt; Pierre-Marc Delaux; Jean-Jacques Bono; Benoit Lefebvre
• Keywords: Lipopolysaccharides; 570; Chitosan; 0303 health sciences; Vegetal Biology; [SDV]Life Sciences [q-bio]; 500; Oligosaccharides; Chitin; Fabaceae; Article; Petunia; 03 medical and health sciences; Solanum lycopersicum; Gene Expression Regulation, Plant; Mycorrhizae; [SDV.BV]Life Sciences [q-bio]/Vegetal Biology; Symbiosis; Protein Kinases; Biologie végétale; Plant Proteins; Rhizobium; Signal Transduction

Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM receptor-like kinases LYK10 are impaired in AM formation. Petunia and tomato LYK10 proteins have a high affinity for LCOs (Kd in the nM range) comparable to that previously reported for a legume LCO receptor essential for the RNS. Interestingly, the tomato and petunia LYK10 promoters, when introduced into a legume, were active in nodules similarly to the promoter of the legume orthologous gene. Moreover, tomato and petunia LYK10 coding sequences restored nodulation in legumes mutated in their orthologs. This combination of genetic and biochemical data clearly pinpoints Solanaceous LYK10 as part of an ancestral LCO perception system involved in AM establishment, which has been directly recruited during evolution of the RNS in legumes.