FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa

• DOI: 10.1016/j.cub.2021.04.060
• Publication Date: 2021-05-19T15:16:21Z
• Authors: Lili Zhang; Jiabao Huang; Shiqi Su; Xiaochun Wei; Lin Yang; Huanhuan Zhao; Jianqiang Yu; Jie Wang; Jiyun Hui; Shiya Hao; Shanshan Song; Yanyan Cao; Maoshuai Wang; Xiaowei Zhang; Yanyan Zhao; Zhiyong Wang; Weiqing Zeng; Hen-Ming Wu; Yuxiang Yuan; Xiansheng Zhang; Alice Y. Cheung; Qiaohong Duan
• Keywords: Brassica rapa

Most plants in the Brassicaceae evolve self-incompatibility (SI) to avoid inbreeding and generate hybrid vigor. Self-pollen is recognized by S-haplotype-specific interaction of pollen ligand S-locus protein 11 (SP11) (also known as cysteine-rich [SCR]) its stigma-specific receptor kinase (SRK). However, mechanistically much remains unknown about signaling events that culminate self-pollen rejection. Here, we show triggers high levels reactive oxygen species (ROS) stigma papilla cells mediate SI heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). We found stigmatic ROS increased after self-pollination but decreased compatible(CP)- pollination. Reducing scavengers or suppressing expression respiratory burst oxidase homologs (Rbohs), which encode plant NADPH oxidases produce ROS, both broke down SI. On other hand, increasing level inhibited germination penetration compatible on stigma, mimicking an incompatible response. Furthermore, a B. FERONIA (FER) homolog Rac/Rop guanosine triphosphatase (GTPase) effectively reduced interfered with Our results suggest FER-Rac/Rop signaling-regulated, oxidase-produced essential response leading