Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling

• DOI: 10.1016/j.devcel.2013.01.027
• Publication Date: 2013-02-28T18:03:31Z
• Authors: Mark R. Morgan; Hellyeh Hamidi; Mark D. Bass; Stacey Warwood; Christoph Ballestrem; Martin J. Humphries
• Keywords: Syndecan 1

Precise spatiotemporal coordination of integrin adhesion complex dynamics is essential for efficient cell migration. For cells adherent to fibronectin, differential engagement α5β1 and αVβ3 integrins used elicit changes in stability, mechanosensation, matrix assembly, migration, but the mechanisms responsible receptor regulation have remained largely obscure. We identify phosphorylation membrane-intercalated proteoglycan syndecan-4 as an switch controlling recycling. Src phosphorylates and, by driving syntenin binding, leads suppression Arf6 activity recycling plasma membrane at expense α5β1. The resultant elevation promotes stabilization focal adhesions. Conversely, abrogation drives surface expression α5β1, destabilizes complexes, disrupts These data dynamic Src-mediated trafficking promote