Midgut Laterality Is Driven by Hyaluronan on the Right

• DOI: 10.1016/j.devcel.2018.08.002
• Publication Date: 2018-08-30T14:54:12Z
• Authors: Aravind Sivakumar; Aparna Mahadevan; Mark E. Lauer; Ricky J. Narvaez; Siddesh Ramesh; Cora M. Demler; Nathan R. Souchet; Vincent C. Hascall; Ron J. Midura; Stavros Garantziotis; David B. Frank; Koji Kimata; Natasza A. Kurpios
• Keywords: Male; Mice, Knockout; Mice, Inbred BALB C; 0303 health sciences; Chick Embryo; Embryo, Mammalian; Functional Laterality; Mice; 03 medical and health sciences; Alpha-Globulins; Animals; Female; Hyaluronic Acid; Cell Adhesion Molecules; Chickens; Digestive System; Body Patterning

For many years, biologists have focused on the role of Pitx2, expressed on the left side of developing embryos, in governing organ laterality. Here, we identify a different pathway during left-right asymmetry initiated by the right side of the embryo. Surprisingly, this conserved mechanism is orchestrated by the extracellular glycosaminoglycan, hyaluronan (HA) and is independent of Pitx2 on the left. Whereas HA is normally synthesized bilaterally as a simple polysaccharide, we show that covalent modification of HA by the enzyme Tsg6 on the right triggers distinct cell behavior necessary to drive the conserved midgut rotation and to pattern gut vasculature. HA disruption in chicken and Tsg6-/- mice results in failure to initiate midgut rotation and perturbs vascular development predisposing to midgut volvulus. Our study leads us to revise the current symmetry-breaking paradigm in vertebrates and demonstrates how enzymatic modification of HA matrices can execute the blueprint of organ laterality.