Active site specificity profiling datasets of matrix metalloproteinases (MMPs) 1, 2, 3, 7, 8, 9, 12, 13 and 14

• DOI: 10.1016/j.dib.2016.02.036
• Publication Date: 2016-02-21T22:15:10Z
• Authors: Ulrich Eckhard; Pitter F. Huesgen; Oliver Schilling; Caroline L. Bellac; Georgina S. Butler; Jennifer H. Cox; Antoine Dufour; Verena Goebeler; Reinhild Kappelhoff; Ulrich auf dem Ke...; Theo Klein; Philipp F. Lange; Giada Marino; Charlotte J. Morr...; Anna Prudova; David Rodriguez; Amanda E. Starr; Yili Wang; Christopher M. Ov...
• Keywords: Cleavage (geology)

The data described provide a comprehensive resource for the family-wide active site specificity portrayal of human matrix metalloproteinase family. We used high-throughput proteomic technique PICS (Proteomic Identification protease Cleavage Sites) to comprehensively assay 9 different MMPs. identified more than 4300 peptide cleavage sites, spanning both prime and non-prime sides scissile bond allowing detailed subsite cooperativity analysis. were expanded by kinetic analysis using set 6 quenched-fluorescent substrates designed these results. These datasets represent one largest profiling efforts with subsequent structural follow up any family put spotlight on similarities differences MMP A this may be found in Eckhard et al. (2015) [1]. raw mass spectrometry corresponding metadata have been deposited PRIDE/ProteomeXchange accession number http://www.ebi.ac.uk/pride/archive/projects/PXD002265.