Increased inflammatory low-density neutrophils in severe obesity and effect of bariatric surgery: Results from case-control and prospective cohort studies

• DOI: 10.1016/j.ebiom.2022.103910
• Publication Date: 2022-03-03T10:03:13Z
• Authors: Maria Dulfary San...; William S. Richar...; Jovanny Zabaleta; Ramesh Thylur Put...; Andrew G. Chapple; Jiao Liu; Yonghyan Kim; Michelle Ponder; Randi DeArmitt; Lyndsey Buckner B...; Dorota Wyczechowska; Liqin Zheng; Amir A. Al-Khami; Jone Garai; Rachel Martini; Melissa Davis; Jessica Koller Go...; James B. Wooldridge; Paulo C. Rodriguez; Lucio Miele; Augusto C. Ochoa
• Keywords: Gene signature

BackgroundLow-density neutrophils (LDN) are increased in several inflammatory diseases and may also play a role the low-grade chronic inflammation associated with obesity. Here we explored their obesity, determined gene signatures, assessed effect of bariatric surgery.MethodsWe compared number, function, expression profiles circulating LDN morbidly obese patients (MOP, n=27; body mass index (BMI) > 40 Kg/m2) normal-weight controls (NWC, n=20; BMI < 25 case-control study. Additionally, prospective longitudinal study, measured changes frequency after surgery (n=36) tested for associations metabolic parameters.FindingsLDN markers were significantly MOP to NWC. Transcriptome analysis showed neutrophil-related signatures inflammation, neutrophil activation, immunosuppressive function. However, did not suppress T cells proliferation produced low levels reactive oxygen species (ROS). Circulating decreased parallel BMI, syndrome, markers.InterpretationObesity increases displaying an signature. Our results suggest that represent subset feature obesity has been previously appearance progression co-morbidities. Furthermore, surgery, as efficient therapy severe reduces circulation improves components syndrome supporting its recognized anti-inflammatory beneficial effects.FundingThis work was supported part by grants from National Institutes Health (NIH; 5P30GM114732-02, P20CA233374 – A. Ochoa L. Miele), Pennington Biomedical NORC (P30DK072476 E. Ravussin & LSU-NO Stanley S. Scott Cancer Center Louisiana Clinical Translational Science (LACaTS; U54-GM104940 J. Kirwan).