Global transmission of extended-spectrum cephalosporin resistance in Escherichia coli driven by epidemic plasmids

• DOI: 10.1016/j.ebiom.2024.105097
• Publication Date: 2024-04-11T18:51:54Z
• Authors: Roxana Zamudio; Patrick Boerlin; Michael R. Mulvey; Marisa Haenni; Racha Beyrouthy; Jean-Yves Madec; Stefan Schwarz; Ashley Cormier; Gabhan Chalmers; Richard Bonnet; George G. Zhanel; Heike Kaspar; Alison E. Mather
• Keywords: 0301 basic medicine; 570; Whole genome sequencing Research; 610; Articles; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; 03 medical and health sciences; ESBL; Whole genome sequencing; 616; Escherichia coli; AMR; AmpC; Epidemic plasmid

Extended-spectrum cephalosporins (ESCs) are third and fourth generation cephalosporin antimicrobials used in humans animals to treat infections due multidrug-resistant (MDR) bacteria. Resistance ESCs (ESC-R) Enterobacterales is predominantly the production of extended-spectrum β-lactamases (ESBLs) plasmid-mediated AmpC (AmpCs). The dynamics ESBLs AmpCs changing across countries host species, result global transmission ESC-R genes. Plasmids known play a key role this dissemination, but relative importance different types plasmids not fully understood. In study, Escherichia coli with major genes blaCTX-M-1, blaCTX-M-15, blaCTX-M-14 blaCMY-2 (AmpC), were selected from diverse species other sources Canada, France Germany, collected between 2003 2017. To examine detail vehicles genes, long- short-read sequences generated obtain complete contiguous chromosome plasmid (n = 192 E. coli). types, gene composition genetic relatedness these investigated, along association isolate year, source geographical origin, put context publicly available sequences. We identified five epidemic resistance subtypes distinct properties that associated dissemination multiple lineages species. IncI1 pST3 blaCTX-M-1 subtype was found more than main subtypes, whereas pST12 frequent Canadian German human chicken isolates. Clonal expansion also contributed ST131 ST117 harbouring plasmid. pST2 predominant isolates France, while IncF F31:A4:B1 blaCTX-M-15 F2:A-:B- cattle countries. Beyond their nature respect our collection almost all carried antimicrobial (AMR) conferring classes. Finally, we signatures regions surrounding specific identifying mechanisms movement, using databases, widespread bacterial continents. provide evidence contribute addition, some confer success suggests they may have fitness advantage over subtypes. Identification understanding AMR crucial develop target strategies interventions reduce spread AMR. This project supported by Joint Programming Initiative on Antimicrobial (JPIAMR), through Medical Research Council (MRC, MR/R000948/1), Institutes Health (CFC-150770), Genomics Development (Government Canada), Federal Ministry Education (BMBF) grant no. 01KI1709, French Agency for food environmental occupational health & safety (Anses), National Reference Center (CNR) resistance. Support provided Biotechnology Biological Sciences (BBSRC) BBSRC Institute Strategic Programme Microbes Food ChainBB/R012504/1 its constituent BBS/E/F/000PR10348 (Theme 1, Epidemiology Evolution Pathogens Chain).