Enhancing stimulation of cyaniding, GhLDOX3 activates reactive oxygen species to regulate tolerance of alkalinity negatively in cotton

• DOI: 10.1016/j.ecoenv.2023.115655
• Publication Date: 2023-11-02T10:52:15Z
• Authors: Tiantian Jiang; Yunxin He; Zhe Wu; Yupeng Cui; Xiuping Wang; Hui Huang; Yapeng Fan; Mingge Han; Junjuan Wang; Shuai Wang; Xiugui Chen; Xuke Lu; Delong Wang; Lixue Guo; Lanjie Zhao; Fushun Hao; Wuwei Ye
• Keywords: Plant Physiology; Wild type

Anthocyanins belong to flavonoid secondary metabolites that act as plant pigments give flowers and fruits different colors "scavengers" of reactive oxygen species (ROS) protect plants from abiotic biotic stresses. Few studies linked anthocyanins alkaline resistance so far. In this study, anthocyanin synthesis-related gene leucoanthocyanidin dioxygenase (LDOX) was screened a candidate explore its relationship with alkali stress. The results found pYL156: GhLDOX3 lines treated 50 mM Na2CO3 (pH 11.11) for 24 h showed significant increase in peroxidase (POD) activity, decrease total content an cyanidin ROS accumulation compared pYL156. overexpressed (OE) lines, ldox mutant wild-type (WT) Arabidopsis were Na2CO3, 100 150 8 d, respectively. wilted degree the OE more severe than WT less treatment. After treatment, expression levels AtCAT AtGSH genes related antioxidant system significantly lower WT, higher WT. conclusion, above suggest played negative regulatory role mechanism resisting Therefore, it can be considered cotton breeding improve tolerance by regulating genes.