Tingenone, a pentacyclic triterpene, induces peripheral antinociception due to NO/cGMP and ATP-sensitive K+ channels pathway activation in mice

• DOI: 10.1016/j.ejphar.2015.02.038
• Publication Date: 2015-03-05T05:00:34Z
• Authors: Clarice de Carval...; Vanessa Gregório ...; Renata Cristina M...; Lucienir Pains Du...; Andre Klein; Igor Dimitri Duarte; Thiago Roberto Li...; Andrea de Castro ...
• Keywords: Channel blocker; Zaprinast; Cyclic guanosine monophosphate; Soluble guanylyl cyclase; Iberiotoxin

Substances derived from plants play an important role in the development of new analgesic drugs, among them, triterpenoids. The connection between participation L-arginine/NO/cGMP pathway and activation ATP-sensitive K(+) channels (KATP) has been established on peripheral antinociception induced by various drugs. study assessed involvement L-arginine/NO/cGMP/KATP antinociceptive effect tingenone, Maytenus imbricata, against hyperalgesia evoked prostaglandin E2 (PGE2) pathway. paw pressure test was used, with intraplantar injection PGE2 (2 μg). Tingenone (200 µg/paw) administered into right hind a local effect, that antagonized l-NOArg, nonselective nitric oxide synthase (NOS) inhibitor L-NPA, selective neuronal NOS (nNOS) inhibitor. L-NIO, endothelial (eNOS), L-NIL, inducible (iNOS), did not alter tingenone. ODQ, soluble guanylyl cyclase inhibitor, prevented zaprinast, phosphodiesterase is cyclic guanosine monophosphate (cGMP) specific, intensified smaller dose Glibenclamide, blocker, but tetraethylammonium chloride, voltage-dependent channel blocker; dequalinium dichloride, blocker small conductance Ca(2+)-activated channel, paxilline, potent high-conductance channels, respectively, results demonstrate tingenone activation, potential for drug.