GPR160 is not a receptor of anorexigenic cocaine- and amphetamine-regulated transcript peptide

• DOI: 10.1016/j.ejphar.2023.175713
• Publication Date: 2023-04-11T15:01:59Z
• Authors: Leandro Ceotto Fr...; Andrea Pačesová; Jana Staňurová; Pavel Šácha; Aleš Marek; Martin Hubálek; Jaroslav Kuneš; Blanka Železná; Lenka Maletínská
• Keywords: Cart; Orphan receptor

Cocaine- and amphetamine-regulated transcript peptide (CARTp) is an anorexigenic neuropeptide whose receptor undisclosed. Previously, we reported the specific binding of CART(61-102) to pheochromocytoma PC12 cells, where affinity number sites per cell corresponded ligand-receptor binding. Recently, Yosten et al. designated orphan GPR160 as CARTp receptor, because antibody abolished neuropathic pain effects induced by CART(55-102) exogenous coimmunoprecipitated with in KATOIII cells. As no direct evidence that a ligand for has been described, decided verify this hypothesis testing receptor. We investigated expression cells since it line known specifically bind CARTp. Moreover, examined THP1 high endogenous GPR160-transfected lines U2OS U-251 MG. In did not compete 125I-CART(61-102) or 125I-CART(55-102), mRNA immunoreactivity were detected. show any 125I-CART(55-102) despite detection fluorescent immunocytochemistry (ICC). Finally, MG, selected due their negligible GPR160, was detected, ICC. Our studies clearly demonstrated cannot be Further are needed identify true receptors.