Berberine modulates gut microbiota to attenuate cerebral ferroptosis induced by ischemia-reperfusion in mice

• DOI: 10.1016/j.ejphar.2023.175782
• Publication Date: 2023-05-26T15:04:13Z
• Authors: Xinyu Wang; Jiamin Zhang; Sisi Wang; Zhengyang Song; Hongxia Sun; Fangquan Wu; Xiaohui Lin; Keke Jin; Xiaofeng Jin; Wantie Wang; Qiongqiong Lin; Fangyan Wang
• Keywords: Bacteroidaceae; Malondialdehyde

Ferroptosis was reported to be involved in cerebral ischemia-reperfusion injury (CIRI), on which the effects of berberine (BBR) remain unclear. Moreover, based critical role gut microbiota pleiotropic actions BBR, we hypothesized that BBR can suppress CIRI-induced ferroptosis by modulating microbiota. In this study, results showed obviously attenuated behavioral deficits CIRI mice, accompanied with improved survival rate and neuron damages, as phenocopied dirty cage experiment. The typical morphological changes ferroptotic cells biomarkers were BBR- its fecal microbiota-treated reduced malondialdehyde reactive oxygen species, increased glutathione (GSH). found alter mice decreased abundance Muribaculaceae, Erysipelotrichaceae, Helicobacteraceae, Streptococcaceae Tannerellaceae, but elevated Bacteroidaceae Enterobacteriaceae. KEGG analysis 16S rRNA indicated multiple metabolic pathways including GSH metabolism, altered BBR. Oppositely, antibiotics administration counteracted protective properties Summarily, study revealed therapeutic potential via inhibiting neuronal ferroptosis, upregulated peroxidase 1 (GPX1) possibly involved. BBR-modulated shown play underlying mechanism.