Hybrid hydrogels for bacteriocin delivery to infected wounds

• DOI: 10.1016/j.ejps.2021.105990
• Publication Date: 2021-09-02T06:05:00Z
• Authors: Raj Kumar Thapa; Hanne Cecilie Win...; Kirill Ovchinnikov; Harald Carlsen; Dzung B. Diep; Hanne Hjorth Tønn...
• Keywords: Methicillin-Resistant Staphylococcus aureus; 0301 basic medicine; Mice; 03 medical and health sciences; Bacteriocins; Wound Infection; Animals; Hydrogels; Staphylococcal Infections; Anti-Bacterial Agents; 3. Good health

Superficial infections in chronic wounds can prevent the wound healing process by development of persistent and drug-resistant biofilms. Topically applied antimicrobial formulations with stabilized controlled release offer significant benefits for effective treatment infections. Bacteriocins are peptides (AMPs) produced bacteria that viable alternatives to antibiotics owing their natural origin low propensity resistance development. Herein, we developed a hybrid hydrogel composed Pluronic F127 (PF127), ethylenediaminetetraacetic acid (EDTA) loaded liposomes, glutathione (GSH), bacteriocin Garvicin KS (GarKS) referred as "GarKS gel". The GarKS gel exhibited suitable viscosity rheological properties along behavior (up 9 days) peptide delivery following topical application. Potent vitro antibacterial anti-biofilm effects were evident against Gram-positive bacterium Staphylococcus aureus. vivo methicillin resistant S. aureus (MRSA) infected mouse suggested potent multiple applications once-a-day application three consecutive days. Altogether, these results provide proof-of-concept successful AMP formulation