Hyaluronan Biodegradable Scaffold for Small-caliber Artery Grafting: Preliminary Results in an Animal Model

• DOI: 10.1016/j.ejvs.2006.02.012
• Publication Date: 2006-05-09T17:20:57Z
• Authors: S. Lepidi; F. Grego; V. Vindigni; B. Zavan; C. Tonello; G.P. Deriu; G. Abatangelo; R. Cortivo
• Keywords: Male; 0301 basic medicine; Biocompatible Materials; Vascular graft; Biomaterials; Rats, Sprague-Dawley; Biomaterials; Peripheral vascular disease; Vascular graft;; Blood Vessel Prosthesis Implantation; 03 medical and health sciences; Absorbable Implants; Animals; Aorta, Abdominal; Hyaluronic Acid; Cells, Cultured; Medicine(all); 0303 health sciences; Tissue Engineering; Arteries; Blood Vessel Prosthesis; Rats; 3. Good health; Peripheral vascular disease; Surgery; Endothelium, Vascular; Cardiology and Cardiovascular Medicine

To evaluate a new Hyaluronan-based graft.Hyaluronan-based grafts (HYAFF 11trade mark tube, diameter 2 mm, length 1.5 cm) were implanted in an end-to-end fashion in the abdominal aorta of 15 rats. Histology, immunohistochemistry and electron microscopy were used to evaluate the results at 7, 21, and 90 days.At day 7, new tissue was observed in the graft coming from both the proximal and distal ends of the aorta. The luminal surface of the regenerating tissue was covered by endothelial cells (CD34(+), VEGFR-2(+), vWF(+)). At day 21, regenerating tissue joined at the centre of the tube. The neo-vessel was formed by smooth muscle cells (Myosin Light Chain Kinase) as well as elastic, and collagen fibres. At day 90 a stable artery segment was formed and the biomaterial was almost completely degraded. Infiltration of neutrophils and lymphocytes was not observed. All animals survived the observation period and there were no signs of stenoses or aneurysms.The hyaluronan-based graft allowed complete regeneration of a newly formed vascular tube in which all the cellular and extracellular components are present and organized in a well defined architecture similar to native artery.