Industrial effluents boosted antibiotic resistome risk in coastal environments

• DOI: 10.1016/j.envint.2022.107714
• Publication Date: 2022-12-22T07:09:27Z
• Authors: Zhiguo Su; Donghui Wen; April Z. Gu; Yuhan Zheng; Yushi Tang; Lyujun Chen
• Keywords: Resistome

Wastewater treatment plants (WWTPs) have been regarded as an important source of antibiotic resistance genes (ARGs) in environment, but out municipal domestic WWTPs, few evidences show how environment is affected by industrial WWTPs. Here we chose Hangzhou Bay (HZB), China our study area, where land-based and WWTPs discharged their effluent into the bay for decades. We adopted high-throughput metagenomic sequencing to examine resistome WWTP coastal sediment samples. And proposed a conceptual framework assessment risk, new bioinformatic pipeline evaluation potential horizontal gene transfer (HGT) frequency. Our results revealed that diversity abundance ARGs WWTP's were significantly higher than those sediment. Furthermore, effluent-receiving area (ERA) showed significant difference from HZB. For first time, identified boosted risk The crucial included: 1) proportion derived activated sludge (WA) was (14.3 %) two high-risky polymyxin (mcr-4 mcr-5) enriched receiving area; 2) HGT between resistant microbiome its ERA sediment; 3) highest determined effluent, some biocide located on contigs related long-term stress chemicals. These findings highlight effects activities development environmental antimicrobial resistance.