Trajectory of neutrophilic responses in a mouse model of pollutant-aggravated allergic asthma

• DOI: 10.1016/j.envpol.2023.121722
• Publication Date: 2023-04-25T15:35:53Z
• Authors: Joyceline De Volder; Annelies Bontinck; Katrien De Grove; Iris Dirven; Valerie Haelterman; Guy Joos; Guy Brusselle; Tania Maes
• Keywords: CXCL1; Interleukin 8

Experimental studies suggest that neutrophils could contribute to allergic asthma pathogenesis, is mainly driven by type 2 immunity. Inhalation of diesel exhaust particles (DEP) implicated in both exacerbation and development asthma. Since exposure DEP associated with a neutrophilic component, we aimed investigate how the combination allergens modulates responses. Human bronchial epithelial cells (HBEC) were exposed house dust mite (HDM), or HDM + vitro determine expression neutrophil-recruiting chemokines. Female (C57BL/6 J) mice intranasally instilled saline, DEP, combined for 3 weeks (subacute) 6 (chronic). The responses determined lung tissue bronchoalveolar lavage fluid (BALF). Simultaneous resulted increased CXCL1 CXCL8 mRNA HBEC vitro. In mice, subacute induced strong mixed eosinophilic/neutrophilic inflammation BALF was higher neutrophil-attracting chemokines NET formation compared sole exposures. After chronic exposure, similar response observed, however less pronounced. Interestingly, increase eosinophils also significantly attenuated after exposure. Subacute goblet cell hyperplasia airway hyperresponsiveness. Our data role NETs pollutant-aggravated eosinophilic Moreover, induces whereas upon there shift inflammatory more prominent component.